Your assignment is to prepare and submit a paper on high let alpha particles. Human chromosomes can undergo structural changes in exposure to high LET alpha particles. The study tries to evaluate the efficacy of three-color FISH and MFish methodology to assess and demarcate these changes. Retired male workers from the Russian Nuclear Industry who were expected to have high exposure to high LET alpha particles during work conditions were involved in the study and standard methodologies were used for the study.
A number of experiments have been reviewed by the author to support the background of the study. Seven studies have been referenced in the paper to support the view that complex aberrations are induced on exposure to high-LET radiation. The definition of insertion which is the characteristic feature of aberrations also has been included in the review. A number of studies involving the FISH technique have profoundly emphasized the importance and relevance of the complexity of radiation-induced chromosome aberrations. The relevance of the fact that the rearranged chromosomes may comprise of parts of multiple chromosomes in the study also has been reviewed. The MFish methodology demonstrated in two different studies one by R. M. Anderson, S. J. Marsden, S. J. Paice, A. E. Bristow, M. A. Kadhim, C. S. Griffin and D. T. Goodhead and another by R. M. Anderson, D. L. Stevens, and D. T. Goodhead have been supportive of this observation. The induction of complex chromosome aberrations in human peripheral blood lymphocytes (PBLs), when exposed to a low dose of high-LET under laboratory conditions, has been supported by different studies and it has been found that the insertions which were visible were parts of other complex aberrations. The references made have supported the background information that the complexity of the aberration induced is much related to the alpha particle exposure. .  .  . .
The study by R. M. Anderson, S. J. Marsden, S. J. Paice, A. E. Bristow, M. A. Kadhim, C. S. Griffin, and D. T. Goodhead have related the in vivo experiment with relevance to the exposure to high-LET particles. It has been opined that long after the exposure, the possibility of efficient detection of stable insertions could be unlikely. The role of Radon, which is a natural emitter of an alpha particle and which is present in the environment has been confirmed by the background information as a consistent source of high LET alpha particle exposure.