Paper instructions:
QUESTION 2 You use FRAP to assess TRPV1 trafficking in cultured neurons expressing GFP-tagged TRPV1. After bleaching GFP-TRPV1 in peripheral nerve terminals, you measure fluorescence recovery overtime, in the following experimental conditions: • Group A: Control, untreated neurons. • Group B: Transgenic neurons with a mutation decreasing the ATPase activity (ATP hydrolysis rate) of KIF 13B • Group C: Neurons with a mutation preventing the binding of the tail of KIF13B to centaurin a1, an adaptor protein required for the transport of TRPV1-containing vesicles. Group A: Control The results are shown here. However, some of the labels are missing. Curve 2 (?) Fluorescence intensity Curve 1 (?) Time 2.1 Which curve (1 or 2) corresponds to Group B? (1 point) Explain why the rate (speed) and extent (full recovery, partial recovery, etc.) of fluorescence recovery differ in this experimental condition, when compared to control neurons. (3 points) 2.2 Which curve (1 or 2) corresponds to Group C? (1 point) Explain why the rate (speed) and extent (full recovery, partial recovery, etc.) of fluorescence recovery differ in this experimental condition, when compared to control neurons. (3 points)
